RNA14/YMR061W Summary

RNA14 BASIC INFORMATION

Standard Name	RNA14
Systematic Name	YMR061W
Feature Type	ORF, Verified
Description	Cleavage and polyadenylation factor I (CF I) component involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex (1, 2)
Name Description	RNA synthesis

GO Annotations	All RNA14 GO evidence and references
	View Computational GO annotations for RNA14
Molecular Function
Manually curated	protein heterodimerization activity (IPI) contributes_to RNA binding (IDA)
Biological Process
Manually curated	mRNA cleavage (IDA) mRNA polyadenylation (IDA)
Cellular Component
Manually curated	mitochondrion (IDA) mRNA cleavage factor complex (IPI) nucleus (IDA)

Mutant Phenotype	All RNA14 Phenotype details and references
Classical genetics
conditional	poly(A)+ RNA accumulation: decreased 3' extended forms of NAB2 mRNA accumulation: increased heat sensitivity: increased snoRNP distribution: abnormal U14 snoRNA distribution: abnormal resistance to cordycepin: decreased vegetative growth: decreased
Large-scale survey
conditional	heat sensitivity: increased
null	competitive fitness: decreased haploinsufficient inviable

Interactions	RNA14 All interactions details and references
	146 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 74 Affinity Capture-RNA: 2 Affinity Capture-Western: 9 Co-fractionation: 1 Far Western: 1 Reconstituted Complex: 4 Two-hybrid: 14
Genetic Interactions	Dosage Rescue: 4 Phenotypic Enhancement: 10 Phenotypic Suppression: 11 Synthetic Growth Defect: 1 Synthetic Lethality: 9 Synthetic Rescue: 6

Sequence Information

ChrXIII:392754 to 394787 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2034	392754..394787	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004665

RNA14 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for RNA14

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RNA14]

1)	Gross S and Moore C (2001) Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I. Proc Natl Acad Sci U S A 98(11):6080-5
2)	Gross S and Moore CL (2001) Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Mol Cell Biol 21(23):8045-55

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