SEN15/YMR059W Summary 
SEN15 BASIC INFORMATION
| Standard Name | SEN15 1 |
|---|---|
| Systematic Name | YMR059W |
| Feature Type | ORF, Verified |
| Description | Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p (1 and see Summary Paragraph) 
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| Name Description | Splicing ENdonuclease 1 |
| Gene Product Alias | tRNA splicing endonuclease subunit 1 |
| GO Annotations | All SEN15 GO evidence and references |
|---|---|
| View Computational GO annotations for SEN15 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Mutant Phenotype | All SEN15 Phenotype details and references |
|---|---|
| Classical genetics | |
| null | |
| Large-scale survey | |
| null | |
| reduction of function |
| Interactions | SEN15 All interactions details and references |
|---|---|
| View additional details at BioGRID | |
| 72 total interaction(s) for 70 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000004663 |
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SEN15 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for SEN15
SUMMARY PARAGRAPH for SEN15
In tRNA splicing, the intron is removed from the tRNA transcript by the tRNA splicing endonuclease; the resulting half-molecules are joined by the tRNA ligase encoded by TRL1 and the splice junction 2'-phosphate is removed by the tRNA 2'-phosphotransferase encoded by TPT1 (reviewed in reference 2). The yeast tRNA splicing endonuclease consists of four subunits, Sen2p, Sen15p, Sen34p, and Sen54p, all of which are essential for viability (1) and which are largely conserved across eukaryotes and archaea (2). Sen34p and Sen2p show sequence similarity to each other and to the catalytic subunits in other organisms (1, 2). A sen34 mutation specifically blocks 3'-splice site cleavage (3), while a sen2 mutation specifically blocks 5'-splice site cleavage (3). Thus it is thought that the endonuclease complex contains two active sites, each responsible for one of the two cleavages (1, 2). The endonuclease has been assumed to be nuclear, however experimental evidence shows that in S. cerevisiae, tRNA splicing occurs in the cytoplasm, with the splicing endonuclease located on the outer surface of the mitochondrial membrane (4, 5), presumably via a hydrophobic segment of Sen2p (1). In contrast, in human cells, the endonuclease has been shown to be localized to the nucleus (6).
Last updated: 2008-09-03
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SEN15]
| 1) | Trotta CR, et al. (1997) The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases. Cell 89(6):849-58 |
| 2) | Calvin K and Li H (2008) RNA-splicing endonuclease structure and function. Cell Mol Life Sci 65(7-8):1176-85 |
| 3) | Ho CK, et al. (1990) Accumulation of pre-tRNA splicing '2/3' intermediates in a Saccharomyces cerevisiae mutant. EMBO J 9(4):1245-52 |
| 4) | Yoshihisa T, et al. (2003) Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria. Mol Biol Cell 14(8):3266-79 |
| 5) | Yoshihisa T, et al. (2007) Cytoplasmic splicing of tRNA in Saccharomyces cerevisiae. Genes Cells 12(3):285-97 |
| 6) | Paushkin SV, et al. (2004) Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation. Cell 117(3):311-21 |
