BUB2/YMR055C Summary Help

BUB2 BASIC INFORMATION

Standard Name BUB2
Systematic Name YMR055C
Alias PAC7
Feature Type ORF, Verified
Description Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage (1, 2, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Budding Uninhibited by Benzimidazole 8
GO Annotations All BUB2 GO evidence and references
    View Computational GO annotations for BUB2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BUB2 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions BUB2 All interactions details and references
170 total interaction(s) for 103 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 12
  • Biochemical Activity: 2
  • Co-localization: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 10
Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 43
  • Phenotypic Suppression: 12
  • Synthetic Growth Defect: 28
  • Synthetic Lethality: 31
  • Synthetic Rescue: 17
Sequence Information
ChrXIII:387020 to 386100 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..921 387020..386100 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004659

BUB2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BUB2

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
CDC16Nomenclature conflictCDC16/YKL022C encodes a subunit of the Anaphase Promoting Complex. Do not confuse CDC16/YKL022C with pombe cdc16, which is similar to the cerevisiae BUB2/YMR055C gene.

SUMMARY PARAGRAPH for BUB2

BUB2 is a non-essential gene whose product is involved in the spindle checkpoint (8, 9). The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules (see 10 for review; reference 11 describes defects detected by the checkpoint). The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators (10). Like other spindle checkpoint mutants, bub2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization (8). Bub2p and Bfa1p localize to the spindle pole body, and appear to act in a branch of the spindle checkpoint pathway that may prevent cytokinesis prior to chromosome segregation (12, 13, 1, 14). Cdh1p, an activator of the anaphase promoting complex, and the protein kinase Dbf2p are likely targets of Bub2p and Bfa1p (12, 13). Genetic evidence suggests that the Bub2p-Bfa1p branch of the spindle checkpoint pathway is distinct from that involving Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and Mps1p, which may prevent premature chromosome disjunction (10, 12, 13, 1, 14).

Last updated: 2000-04-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BUB2]

1) Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
2) Pereira G, et al.  (2000) The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol Cell 6(1):1-10
3) Krishnan R, et al.  (2000) Saccharomyces cerevisiae BUB2 prevents mitotic exit in response to both spindle and kinetochore damage. Genetics 156(2):489-500
4) Hu F, et al.  (2001) Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 107(5):655-65
5) Pereira G, et al.  (2001) Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex. EMBO J 20(22):6359-70
6) Pereira G, et al.  (2002) Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. J Cell Biol 157(3):367-79
7) Molk JN, et al.  (2004) The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit. Mol Biol Cell 15(4):1519-32
8) Hoyt MA, et al.  (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66(3):507-17
9) Roberts BT, et al.  (1994) The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol 14(12):8282-91
10) Hardwick KG  (1998) The spindle checkpoint. Trends Genet 14(1):1-4
11) Hardwick KG, et al.  (1999) Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics 152(2):509-18
12) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
13) Fesquet D, et al.  (1999) A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J 18(9):2424-34
14) Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94