CSM3/YMR048W Summary Help

Standard Name CSM3 1
Systematic Name YMR048W
Feature Type ORF, Verified
Description Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress (1, 2, 3, 4, 5)
Name Description Chromosome Segregation in Meiosis 1
Chromosomal Location
ChrXIII:366981 to 367934 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CSM3 GO evidence and references
  View Computational GO annotations for CSM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
473 total interaction(s) for 216 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 19
  • Biochemical Activity: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 168
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 7
  • Positive Genetic: 11
  • Synthetic Growth Defect: 161
  • Synthetic Lethality: 60
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 317
Molecular Weight (Da) 36,354
Isoelectric Point (pI) 4.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:366981 to 367934 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..954 366981..367934 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004651
References cited on this page View Complete Literature Guide for CSM3
1) Rabitsch KP, et al.  (2001) A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol 11(13):1001-9
2) Calzada A, et al.  (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19(16):1905-19
3) Mohanty BK, et al.  (2006) The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 103(4):897-902
4) Bando M, et al.  (2009) Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks. J Biol Chem 284(49):34355-65
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76