SUB1/YMR039C Summary Help

Standard Name SUB1 1
Systematic Name YMR039C
Alias TSP1
Feature Type ORF, Verified
Description Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description SUppressor of TFIIB mutations 1
Chromosomal Location
ChrXIII:349522 to 348644 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SUB1 GO evidence and references
  View Computational GO annotations for SUB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
232 total interaction(s) for 180 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-localization: 4
  • Far Western: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Negative Genetic: 123
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 5
  • Positive Genetic: 14
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 6
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 292
Molecular Weight (Da) 33,102
Isoelectric Point (pI) 5.4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:349522 to 348644 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..879 349522..348644 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004642
References cited on this page View Complete Literature Guide for SUB1
1) Knaus R, et al.  (1996) Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBO J 15(8):1933-40
2) Wang JY, et al.  (2004) The single-strand DNA binding activity of human PC4 prevents mutagenesis and killing by oxidative DNA damage. Mol Cell Biol 24(13):6084-93
3) Calvo O and Manley JL  (2005) The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription. EMBO J 24(5):1009-20
4) Rosonina E, et al.  (2009) Sub1 Functions in Osmoregulation and in Transcription by both RNA Polymerases II and III. Mol Cell Biol 29(8):2308-21
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Yu L and Volkert MR  (2013) Differential requirement for SUB1 in chromosomal and plasmid double-strand DNA break repair. PLoS One 8(3):e58015