CCS1/YMR038C Summary Help

Standard Name CCS1 1
Systematic Name YMR038C
Alias LYS7 2
Feature Type ORF, Verified
Description Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress (3, 4, 5, 6)
Also known as: CCS
Name Description Copper Chaperone for SOD1 2
Chromosomal Location
ChrXIII:348260 to 347511 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 30 cM
Gene Ontology Annotations All CCS1 GO evidence and references
  View Computational GO annotations for CCS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
Large-scale survey
258 total interaction(s) for 178 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 2
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 85
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 16
  • Synthetic Growth Defect: 99
  • Synthetic Lethality: 41
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 249
Molecular Weight (Da) 27,330
Isoelectric Point (pI) 6.65
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:348260 to 347511 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 30 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..750 348260..347511 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004641
References cited on this page View Complete Literature Guide for CCS1
1) Lamb AL, et al.  (2000) Heterodimer formation between superoxide dismutase and its copper chaperone. Biochemistry 39(48):14720-7
2) Culotta VC, et al.  (1997) The copper chaperone for superoxide dismutase. J Biol Chem 272(38):23469-72
3) Schmidt PJ, et al.  (1999) A gain of superoxide dismutase (SOD) activity obtained with CCS, the copper metallochaperone for SOD1. J Biol Chem 274(52):36952-6
4) Brown NM, et al.  (2004) Oxygen and the copper chaperone CCS regulate posttranslational activation of Cu,Zn superoxide dismutase. Proc Natl Acad Sci U S A 101(15):5518-23
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6) Dong K, et al.  (2013) The yeast copper response is regulated by DNA damage. Mol Cell Biol 33(20):4041-50