HOF1/YMR032W Summary Help

Standard Name HOF1 1
Systematic Name YMR032W
Alias CYK2 2
Feature Type ORF, Verified
Description SH3 domain-containing protein required for cytokinesis; localized to bud neck; phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p (1, 2, 3, 4, 5, 6)
Name Description Homolog Of cdc Fifteen 1
Chromosomal Location
ChrXIII:335298 to 337307 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All HOF1 GO evidence and references
  View Computational GO annotations for HOF1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 16 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
null
overexpression
Resources
544 total interaction(s) for 410 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-Western: 4
  • Biochemical Activity: 5
  • Co-localization: 3
  • PCA: 11
  • Reconstituted Complex: 8
  • Two-hybrid: 73

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 87
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 4
  • Positive Genetic: 292
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 20
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 669
Molecular Weight (Da) 76,206
Isoelectric Point (pI) 9.85
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:335298 to 337307 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2010 335298..337307 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004635
References cited on this page View Complete Literature Guide for HOF1
1) Kamei T, et al.  (1998) Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae. J Biol Chem 273(43):28341-5
2) Lippincott J and Li R  (1998) Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution. J Cell Biol 143(7):1947-60
3) Korinek WS, et al.  (2000) Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Curr Biol 10(15):947-50
4) Naqvi SN, et al.  (2001) Vrp1p functions in both actomyosin ring-dependent and Hof1p-dependent pathways of cytokinesis. Traffic 2(3):189-201
5) Lee PR, et al.  (2002) Bni5p, a septin-interacting protein, is required for normal septin function and cytokinesis in Saccharomyces cerevisiae. Mol Cell Biol 22(19):6906-20
6) Meitinger F, et al.  (2013) Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell 24(9):1290-304