EIS1/YMR031C Summary Help

Standard Name EIS1 1
Systematic Name YMR031C
Feature Type ORF, Verified
Description Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description EISosome 1
Chromosomal Location
ChrXIII:334743 to 332212 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All EIS1 GO evidence and references
  View Computational GO annotations for EIS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 19 genes
Resources
Large-scale survey
null
Resources
78 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 42
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-localization: 3
  • Co-purification: 1
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 18
  • Positive Genetic: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 843
Molecular Weight (Da) 93,344
Isoelectric Point (pI) 6.28
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:334743 to 332212 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2532 334743..332212 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004633
References cited on this page View Complete Literature Guide for EIS1
1) Aguilar PS, et al.  (2010) A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nat Struct Mol Biol 17(7):901-8
2) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
3) Balakrishnan R, et al.  (2005) Fungal BLAST and Model Organism BLASTP Best Hits: new comparison resources at the Saccharomyces Genome Database (SGD). Nucleic Acids Res 33 Database Issue:D374-7
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
6) Deng C, et al.  (2009) Unifying fluorescence microscopy and mass spectrometry for studying protein complexes in cells. Mol Cell Proteomics 8(6):1413-23
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76