SEC59/YMR013C Summary Help

Standard Name SEC59 1, 2
Systematic Name YMR013C
Feature Type ORF, Verified
Description Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation (1, 3 and see Summary Paragraph)
Name Description SECretory 1
Chromosomal Location
ChrXIII:296738 to 295179 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 3 cM
Gene Ontology Annotations All SEC59 GO evidence and references
  View Computational GO annotations for SEC59
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All SEC59 Phenotype evidence and references
Classical genetics
conditional
reduction of function
Large-scale survey
null
reduction of function
Resources
interactions All SEC59 Interaction evidence and references
22 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • PCA: 1
Genetic Interactions
  • Dosage Rescue: 5
  • Negative Genetic: 8
  • Phenotypic Suppression: 1
  • Positive Genetic: 4
  • Synthetic Lethality: 3
Resources
Expression Summary
histogram
Resources
Protein Information All SEC59 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:296738 to 295179 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YMR013C-A
SGD ORF map
Genetic position: 3 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1560 296738..295179 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004615
SUMMARY PARAGRAPH for SEC59

Dolichols are a class of polyisoprenoid alcohols with five or more isoprene units in which the C2-C3 bond is saturated. In yeast, dolichols with 14-18 isoprene units are the carrier molecules on which many of the steps in N-linked glycosylation, O-linked glycosylation, and GPI anchor synthesis occur on the membrane of the endoplasmic reticulum (ER) (reviewed in 4). In mammals, dolichols with 19-22 isoprene units are the carrier molecules on which these steps occur.

SEC59 encodes dolichol kinase (3), which synthesizes dolichyl-phosphate (Dol-P), the substrate on which oligosaccharide cores are assembled on the ER membrane. Mutants lacking Sec59p produce poorly N-glycosylated proteins that accumulate in the ER, hence the secretory phenotype (5). Dol-P is also used to shuttle mannose (and glucose) into the ER, so sec59 mutants are also deficient in O-linked glycosylation and GPI anchor synthesis (6, 7). (Refer to the entries for DPM1 and ALG5 for information on shuttling mannose and glucose, respectively, into the ER.) A putative human dolichol kinase cDNA complements the sec59-1 mutation (8).

Last updated: 2005-07-01

References cited on this page View Complete Literature Guide for SEC59
1) Bernstein M, et al.  (1989) Sec59 encodes a membrane protein required for core glycosylation in Saccharomyces cerevisiae. Mol Cell Biol 9(3):1191-9
2) Fields, C. and Schekman, R.  (1985) Personal Communication, Mortimer Map Edition 9
3) Heller L, et al.  (1992) Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity. Proc Natl Acad Sci U S A 89(15):7013-6
4) Grabinska K and Palamarczyk G  (2002) Dolichol biosynthesis in the yeast Saccharomyces cerevisiae: an insight into the regulatory role of farnesyl diphosphate synthase. FEMS Yeast Res 2(3):259-65
5) Ferro-Novick S, et al.  (1984) Yeast secretory mutants that block the formation of active cell surface enzymes. J Cell Biol 98(1):35-43
6) Orlean P  (1990) Dolichol phosphate mannose synthase is required in vivo for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and N glycosylation of protein in Saccharomyces cerevisiae. Mol Cell Biol 10(11):5796-805
7) Orlean P  (1992) Enzymes that recognize dolichols participate in three glycosylation pathways and are required for protein secretion. Biochem Cell Biol 70(6):438-47
8) Fernandez F, et al.  (2002) Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae. Glycobiology 12(9):555-62