ERO1/YML130C Summary Help

Standard Name ERO1 1, 2
Systematic Name YML130C
Feature Type ORF, Verified
Description Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds (1, 2, 3, 4)
Name Description ER Oxidation or Endoplasmic Reticulum Oxidoreductin 1, 2
Chromosomal Location
ChrXIII:13174 to 11483 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ERO1 GO evidence and references
  View Computational GO annotations for ERO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 12 genes
Resources
Classical genetics
conditional
misexpression
reduction of function
Large-scale survey
conditional
null
overexpression
Resources
57 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-Western: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 30
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 563
Molecular Weight (Da) 65,032
Isoelectric Point (pI) 5.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:13174 to 11483 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1692 13174..11483 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004599
References cited on this page View Complete Literature Guide for ERO1
1) Frand AR and Kaiser CA  (1998) The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum. Mol Cell 1(2):161-70
2) Pollard MG, et al.  (1998) Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum. Mol Cell 1(2):171-82
3) Gross E, et al.  (2006) Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p. Proc Natl Acad Sci U S A 103(2):299-304
4) Kim S, et al.  (2012) Balanced Ero1 activation and inactivation establishes ER redox homeostasis. J Cell Biol 196(6):713-25