PHO84/YML123C Summary

PHO84 BASIC INFORMATION

Standard Name	PHO84
Systematic Name	YML123C
Feature Type	ORF, Verified
Description	High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p (1, 2, 3, 4) Also known as: phoT 5
Name Description	PHOsphate metabolism

GO Annotations	All PHO84 GO evidence and references
	View Computational GO annotations for PHO84
Molecular Function
Manually curated	inorganic phosphate transmembrane transporter activity (IDA, IMP, ISS) manganese ion transmembrane transporter activity (IMP)
Biological Process
Manually curated	manganese ion transport (IMP) phosphate transport (IDA, IMP, ISS) polyphosphate metabolic process (IMP)
Cellular Component
Manually curated	integral to plasma membrane (IDA, ISS)

Mutant Phenotype	All PHO84 Phenotype details and references
Classical genetics
conditional	acid phosphatase activity: increased
null	manganese accumulation: decreased cobalt accumulation: decreased zinc accumulation: decreased copper accumulation: decreased polyphosphate accumulation: decreased metal resistance: increased nutrient uptake: absent resistance to arsenate(3-): decreased resistance to rapamycin: increased resistance to disodium selenite: decreased resistance to quinine: increased small molecule transport: decreased rate vegetative growth: absent
reduction of function	acid phosphatase activity: increased
Large-scale survey
null	competitive fitness: decreased fermentative growth: decreased rate resistance to rapamycin: decreased resistance to wortmannin: normal resistance to calcium dichloride: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased utilization of phosphorus source: decreased viable

Interactions	PHO84 All interactions details and references
	53 total interaction(s) for 37 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10 Protein-RNA: 1 Two-hybrid: 18
Genetic Interactions	Dosage Rescue: 8 Phenotypic Enhancement: 4 Phenotypic Suppression: 5 Synthetic Growth Defect: 1 Synthetic Lethality: 4 Synthetic Rescue: 2

Sequence Information

ChrXIII:25801 to 24038 | |

Note: this feature is encoded on the Crick strand.

Genetic position: -100.5 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1764	25801..24038	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| TCDB \| UniProtKB

Primary SGDID	S000004592

PHO84 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PHO84

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PHO84]

1)	Bun-ya M, et al. (1996) Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr Genet 29(4):344-51
2)	Nishimura K, et al. (1999) Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by spt7p. Yeast 15(16):1711-7
3)	Bun-Ya M, et al. (1991) The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol 11(6):3229-38
4)	Jensen LT, et al. (2003) The Saccharomyces cerevisiae high affinity phosphate transporter encoded by PHO84 also functions in manganese homeostasis. J Biol Chem 278(43):42036-40
5)	Ueda Y, et al. (1975) Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol 122(3):911-22

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