SEC65/YML105C Summary

SEC65 BASIC INFORMATION

Standard Name	SEC65 1
Systematic Name	YML105C
Feature Type	ORF, Verified
Description	Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 (1, 2 and see Summary Paragraph)
Name Description	SECretory 1

GO Annotations	All SEC65 GO evidence and references
	View Computational GO annotations for SEC65
Molecular Function
Manually curated	7S RNA binding (IDA)
Biological Process
Manually curated	protein targeting to ER (IMP) SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition (IMP)
Cellular Component
Manually curated	signal recognition particle, endoplasmic reticulum targeting (IDA, IMP, ISS)

Mutant Phenotype	All SEC65 Phenotype details and references
Classical genetics
null	SCR1 RNA accumulation: normal growth in exponential phase: decreased rate heat sensitivity: increased nuclear export: normal dipeptidyl-aminopeptidase B secretion: decreased pre-pro-alpha factor secretion: decreased Kar2p accumulation: abnormal carboxypeptidase Y (CPY) accumulation: normal viable
repressible	Pmp2GFP-KKTN distribution: decreased
Large-scale survey
null	inviable
repressible	mitochondrial morphology: abnormal Pmp2GFP-LRKR distribution: decreased

Interactions	SEC65 All interactions details and references
	55 total interaction(s) for 25 unique genes/features.
Physical Interactions	Affinity Capture-MS: 31 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Co-purification: 10
Genetic Interactions	Dosage Rescue: 2 Phenotypic Enhancement: 8

Sequence Information

ChrXIII:58687 to 57866 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..822	58687..57866	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004573

SEC65 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SEC65

SUMMARY PARAGRAPH for SEC65

The signal recognition particle (SRP) is an abundant and conserved ribonucleoprotein necessary for targeting proteins to the endoplasmic reticulum membrane (3). SRP in eukaryotes contains six subunits and a 7S RNA molecule; in S. cerevisiae the subunits are encoded by SRP14, SRP21, SRP68, SRP72, SEC65, and SRP54, and the RNA (termed scR1) is encoded by SCR1 (3, 4). With the exception of Srp54p, the proteins and RNA assemble into a core complex in the nucleus; this particle is exported to the cytoplasm where Srp54p joins to form the complete complex (5). Sec65p is required for association of Srp54p with the SRP particle (2). Loss of any of the SRP components causes a slow growth phenotype and loss of SRP-mediated translocation, but not cell death, indicating that the signal recognition particle is not essential in yeast and SRP-independent translocation can occur (3, 4).

The first step of SRP-mediated cotranslational targeting is interaction between SRP and the ribosome nascent chain complex (RNC), which is comprised of the translating ribosome and the emerging nascent protein. SRP interacts with the RNC through the N-terminal hydrophobic signal sequence of the nascent protein. SRP then directs the RNC to the ER membrane via interaction between SRP and a signal receptor complex (SR), encoded by SRP101 and SRP102. Finally, the RNC is transferred to the translocon, a protein-conducting membrane channel, and SRP and the SR dissociate. GTP binding by both SRP (via the Srp54p subunit) and the SR is critical for their interaction, and GTP hydrolysis facilitates their dissociation (reviewed in 6, and see 6 for more details).

Last updated: 2008-08-11

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SEC65]

1)	Hann BC, et al. (1992) SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity. Nature 356(6369):532-3
2)	Stirling CJ and Hewitt EW (1992) The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356(6369):534-7
3)	Hann BC and Walter P (1991) The signal recognition particle in S. cerevisiae. Cell 67(1):131-44
4)	Brown JD, et al. (1994) Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. EMBO J 13(18):4390-400
5)	Ciufo LF and Brown JD (2000) Nuclear export of yeast signal recognition particle lacking Srp54p by the Xpo1p/Crm1p NES-dependent pathway. Curr Biol 10(20):1256-64
6)	Wild K, et al. (2004) SRP meets the ribosome. Nat Struct Mol Biol 11(11):1049-53

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