VPS9/YML097C Summary Help

Standard Name VPS9 1, 2, 3
Systematic Name YML097C
Alias VPL31 4 , VPT9 1
Feature Type ORF, Verified
Description Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a VPS9 domain; binds ubiquitin (3, 5, 6, 7, 8)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXIII:79690 to 78335 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VPS9 GO evidence and references
  View Computational GO annotations for VPS9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
408 total interaction(s) for 248 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 246
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 54
  • Positive Genetic: 34
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 451
Molecular Weight (Da) 52,482
Isoelectric Point (pI) 4.82
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:79690 to 78335 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1356 79690..78335 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004563
References cited on this page View Complete Literature Guide for VPS9
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Burd CG, et al.  (1996) A yeast protein related to a mammalian Ras-binding protein, Vps9p, is required for localization of vacuolar proteins. Mol Cell Biol 16(5):2369-77
4) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
5) Hama H, et al.  (1999) Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport. J Biol Chem 274(21):15284-91
6) Davies BA, et al.  (2003) Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. J Biol Chem 278(22):19826-33
7) Paulsel AL, et al.  (2013) Vps9 family protein muk1 is the second rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem 288(25):18162-71
8) Cabrera M, et al.  (2013) Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem 288(7):5166-75