BET5/YML077W Summary Help

Standard Name BET5 1
Systematic Name YML077W
Feature Type ORF, Verified
Description Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII) (2, 3, 4, 5)
Name Description Blocked Early in Transport 6
Chromosomal Location
ChrXIII:111865 to 112344 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BET5 GO evidence and references
  View Computational GO annotations for BET5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
repressible
Large-scale survey
conditional
null
reduction of function
Resources
79 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Rescue: 12
  • Negative Genetic: 14
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 159
Molecular Weight (Da) 18,434
Isoelectric Point (pI) 9.26
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:111865 to 112344 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..480 111865..112344 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004542
References cited on this page View Complete Literature Guide for BET5
1) Jiang Y, et al.  (1998) A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport. Genetics 149(2):833-41
2) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
3) Sacher M, et al.  (2001) TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport. Mol Cell 7(2):433-42
4) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6
5) Shirahama-Noda K, et al.  (2013) TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy. J Cell Sci 126(Pt 21):4963-73
6) Newman AP and Ferro-Novick S  (1987) Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol 105(4):1587-94