FPR3/YML074C Summary Help

Standard Name FPR3 1
Systematic Name YML074C
Alias NPI46 2
Feature Type ORF, Verified
Description Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication (2, 3, 4, 5, 6)
Name Description Fk 506-sensitive Proline Rotamase 3
Chromosomal Location
ChrXIII:121324 to 120089 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All FPR3 GO evidence and references
  View Computational GO annotations for FPR3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
88 total interaction(s) for 71 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 47
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Biochemical Activity: 6
  • Co-purification: 2
  • PCA: 3
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 4
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 411
Molecular Weight (Da) 46,553
Isoelectric Point (pI) 4.19
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:121324 to 120089 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1236 121324..120089 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004539
References cited on this page View Complete Literature Guide for FPR3
1) Manning-Krieg UC, et al.  (1994) Purification of FKBP-70, a novel immunophilin from Saccharomyces cerevisiae, and cloning of its structural gene, FPR3. FEBS Lett 352(1):98-103
2) Shan X, et al.  (1994) Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus. J Cell Biol 126(4):853-62
3) Benton BM, et al.  (1994) A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus. J Cell Biol 127(3):623-39
4) Wilson LK, et al.  (1997) Casein kinase II catalyzes tyrosine phosphorylation of the yeast nucleolar immunophilin Fpr3. J Biol Chem 272(20):12961-7
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Park SK, et al.  (2014) Nuclear FKBPs, Fpr3 and Fpr4 affect genome-wide genes transcription. Mol Genet Genomics 289(2):125-36