Standard Name	TCB3 1
Systematic Name	YML072C
Feature Type	ORF, Verified
Description	Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact (2, 3, 4, 5, 6)
Name Description	Three Calcium and lipid Binding domains (TriCalBins) 1

Chromosomal Location	ChrXIII:129367 to 124730 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for TCB3
Molecular Function
Manually curated	lipid binding (IDA)
Biological Process
Manually curated	endoplasmic reticulum membrane organization (IGI) regulation of phosphatidylinositol dephosphorylation (IGI)
Cellular Component
Manually curated	cellular bud (IDA) cortical endoplasmic reticulum (IDA) NOT plasma membrane (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	chronological lifespan: increased resistance to sirolimus: increased resistance to myriocin: decreased viable
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased resistance to fluconazole: increased resistance to caffeine: increased resistance to cycloheximide: increased resistance to bleomycin: increased resistance to PM02734: increased resistance to hydroxyurea: increased resistance to tunicamycin: increased resistance to camptothecin: increased resistance to L-1,4-dithiothreitol: decreased resistance to tunicamycin: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	97 total interaction(s) for 88 unique genes/features.
Physical Interactions	Affinity Capture-MS: 28 Affinity Capture-RNA: 3 Affinity Capture-Western: 2 PCA: 41 Two-hybrid: 3
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 13 Phenotypic Enhancement: 1 Positive Genetic: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:129367 to 124730 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..4638 129367..124730 1996-07-31 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004537