SML1/YML058W Summary Help

Standard Name SML1 1
Systematic Name YML058W
Feature Type ORF, Verified
Description Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication (2, 3, 4)
Name Description Suppressor of Mec1 Lethality 1
Chromosomal Location
ChrXIII:159383 to 159697 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SML1 GO evidence and references
  View Computational GO annotations for SML1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
176 total interaction(s) for 94 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 31
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 2
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 16
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 12
  • Synthetic Rescue: 51

Resources
Expression Summary
histogram
Resources
Length (a.a.) 104
Molecular Weight (Da) 11,834
Isoelectric Point (pI) 4.55
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:159383 to 159697 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..315 159383..159697 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004523
References cited on this page View Complete Literature Guide for SML1
1) Zhao X, et al.  (1998) A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools. Mol Cell 2(3):329-40
2) Chabes A, et al.  (1999) Yeast Sml1, a protein inhibitor of ribonucleotide reductase. J Biol Chem 274(51):36679-83
3) Zhao X and Rothstein R  (2002) The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1. Proc Natl Acad Sci U S A 99(6):3746-51
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61