APT1/YML022W Summary Help

Standard Name APT1 1
Systematic Name YML022W
Feature Type ORF, Verified
Description Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication (2, 3, 4 and see Summary Paragraph)
Name Description Adenine PhosphoribosylTransferase 1
Chromosomal Location
ChrXIII:228937 to 229500 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All APT1 GO evidence and references
  View Computational GO annotations for APT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 7 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
31 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-Western: 1
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 4
  • Phenotypic Enhancement: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 187
Molecular Weight (Da) 20,587
Isoelectric Point (pI) 4.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:228937 to 229500 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..564 228937..229500 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004484
SUMMARY PARAGRAPH for APT1

APT1 encodes the yeast adenine phosphoribosyltransferase (APRT), which catalyzes the condensation of adenine and 5-phosphoribosylpyrophosphate (PRPP) to yield AMP (5, 2, 1). Apt1p has strong similarity to APRT in bacteria, invertebrates, plants, and mammals (1). APT2, a gene that was thought to encode a second yeast APRT, has been found to be a nonfunctional pseudogene that likely arose from a duplication of APT1 (6). Some cases of urolithiasis are caused by mutations in the human APT1 homolog (7).

Last updated: 1999-12-31 Contact SGD

References cited on this page View Complete Literature Guide for APT1
1) Alfonzo JD, et al.  (1995) Cloning and characterization of the adenine phosphoribosyltransferase-encoding gene (APT1) from Saccharomyces cerevisiae. Gene 161(1):81-5
2) Alfonzo JD, et al.  (1997) Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae. Biochim Biophys Acta 1341(2):173-82
3) Deeley MC  (1992) Adenine deaminase and adenine utilization in Saccharomyces cerevisiae. J Bacteriol 174(10):3102-10
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Woods RA, et al.  (1984) Adenine phosphoribosyltransferase mutants in Saccharomyces cerevisiae. J Gen Microbiol 130(10):2629-37
6) Alfonzo JD, et al.  (1999) APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae. J Bacteriol 181(1):347-52
7) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10