TRM9/YML014W Summary Help

Standard Name TRM9 1
Systematic Name YML014W
Alias KTI1 2
Feature Type ORF, Verified
Description tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses (1, 3, 4, 5)
Name Description TRna Methyltransferase 1
Chromosomal Location
ChrXIII:243225 to 244064 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM9 GO evidence and references
  View Computational GO annotations for TRM9
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
76 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 35
  • Phenotypic Suppression: 2
  • Positive Genetic: 14
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 279
Molecular Weight (Da) 32,438
Isoelectric Point (pI) 8.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:243225 to 244064 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..840 243225..244064 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004476
References cited on this page View Complete Literature Guide for TRM9
1) Kalhor HR and Clarke S  (2003) Novel methyltransferase for modified uridine residues at the wobble position of tRNA. Mol Cell Biol 23(24):9283-92
2) Butler AR, et al.  (1991) Intracellular expression of Kluyveromyces lactis toxin gamma subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant. Yeast 7(6):617-25
3) Jablonowski D, et al.  (2006) tRNAGlu wobble uridine methylation by Trm9 identifies Elongator's key role for zymocin-induced cell death in yeast. Mol Microbiol 59(2):677-88
4) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77
5) Patil A, et al.  (2012) Translational infidelity-induced protein stress results from a deficiency in Trm9-catalyzed tRNA modifications. RNA Biol 9(7):990-1001