SPT5/YML010W Summary Help

Standard Name SPT5 1
Systematic Name YML010W
Feature Type ORF, Verified
Description Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrXIII:247677 to 250868 | ORF Map | GBrowse
Genetic position: -5 cM
Gene Ontology Annotations All SPT5 GO evidence and references
  View Computational GO annotations for SPT5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 71 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
328 total interaction(s) for 167 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 216
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 28
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 12
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 14
  • Synthetic Rescue: 13

Expression Summary
Length (a.a.) 1,063
Molecular Weight (Da) 115,649
Isoelectric Point (pI) 4.95
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:247677 to 250868 | ORF Map | GBrowse
Genetic position: -5 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3192 247677..250868 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004470
References cited on this page View Complete Literature Guide for SPT5
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78
3) Rondon AG, et al.  (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20
4) Schneider DA, et al.  (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12
5) Zhou K, et al.  (2009) Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5. Proc Natl Acad Sci U S A 106(17):6956-61
6) Liu Y, et al.  (2009) Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol 29(17):4852-63
7) Ding B, et al.  (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26
8) Shen Z, et al.  (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26
9) Anderson SJ, et al.  (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24
10) Lepore N and Lafontaine DL  (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962