SPT5/YML010W Summary Help

Standard Name SPT5 1
Systematic Name YML010W
Feature Type ORF, Verified
Description Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrXIII:247677 to 250868 | ORF Map | GBrowse
Genetic position: -5 cM
Gene Ontology Annotations All SPT5 GO evidence and references
  View Computational GO annotations for SPT5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
336 total interaction(s) for 168 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 217
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 33
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 13
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 5
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 14
  • Synthetic Rescue: 13

Expression Summary
Length (a.a.) 1,063
Molecular Weight (Da) 115,649
Isoelectric Point (pI) 4.95
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:247677 to 250868 | ORF Map | GBrowse
Genetic position: -5 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3192 247677..250868 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004470
References cited on this page View Complete Literature Guide for SPT5
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78
3) Rondon AG, et al.  (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20
4) Schneider DA, et al.  (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12
5) Zhou K, et al.  (2009) Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5. Proc Natl Acad Sci U S A 106(17):6956-61
6) Liu Y, et al.  (2009) Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex. Mol Cell Biol 29(17):4852-63
7) Ding B, et al.  (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26
8) Shen Z, et al.  (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26
9) Anderson SJ, et al.  (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24
10) Lepore N and Lafontaine DL  (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962