CRN1/YLR429W Summary Help

Standard Name CRN1 1
Systematic Name YLR429W
Feature Type ORF, Verified
Description Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly (2, 3, 4 and see Summary Paragraph)
Name Description CoRoNin 1, 2
Chromosomal Location
ChrXII:990777 to 992732 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CRN1 GO evidence and references
  View Computational GO annotations for CRN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
156 total interaction(s) for 129 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 94
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 5
  • Co-fractionation: 3
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 9

Genetic Interactions
  • Negative Genetic: 16
  • Phenotypic Enhancement: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 651
Molecular Weight (Da) 72,553
Isoelectric Point (pI) 5.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:990777 to 992732 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1956 990777..992732 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004421
SUMMARY PARAGRAPH for CRN1

CRN1, which encodes the yeast homologue of coronin, is an actin- and microtubule-associated protein (1). Crn1p contains five amino-terminal WD repeats which form a beta-propeller structure; a microtubule binding domain; and a carboxyl terminal alpha-helical coiled coil structure. Localization of Crn1p to cortical patches requires both its beta-propeller and coiled-coil domains (5, 6).

Crn1p binds actin filaments (F-actin) and cross-links F-actin to form long actin filament bundles (1). Crn1p also regulates the actin filament nucleation and branching activity of the Arp2/3 complex through interaction with the Arc35p subunit (3). The Arp2/3 complex by itself has weak actin nucleation activity and is activated by binding to the side of an actin filament or by association with activator proteins like Las17p and Abp1p (7). Crn1p inhibits de novo actin nucleation by the Las17p- and Abp1p-activated Arp2/3 complex by binding to the Arc35p subunit which stabilizes an open, inactive conformation of the Arp2/3 complex. This inhibitory role is observed only in the absence of preformed actin filaments. In the presence of preformed filaments, Crn1p restricts the activated-Arp2/3 complex to the sides of actin filaments and permits actin nucleation (3, 2, 8). crn1 null mutants display slow movement of actin cortical patches from the membrane into the cytoplasm (4) while overexpression of CRN1 results in defects in actin and microtubule organization (2).

Coronins are an evoluntionarily well conserved family of WD repeat proteins involved in actin cytoskeleton organization. The C. elegans POD-1 has also been shown to be involved in vesicular trafficking (reviewed in 6).

Last updated: 2008-03-07 Contact SGD

References cited on this page View Complete Literature Guide for CRN1
1) Heil-Chapdelaine RA, et al.  (1998) The role of Saccharomyces cerevisiae coronin in the actin and microtubule cytoskeletons. Curr Biol 8(23):1281-4
2) Goode BL, et al.  (1999) Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast. J Cell Biol 144(1):83-98
3) Humphries CL, et al.  (2002) Direct regulation of Arp2/3 complex activity and function by the actin binding protein coronin. J Cell Biol 159(6):993-1004
4) Galletta BJ, et al.  (2008) Distinct roles for Arp2/3 regulators in actin assembly and endocytosis. PLoS Biol 6(1):e1
5) Moseley JB and Goode BL  (2006) The yeast actin cytoskeleton: from cellular function to biochemical mechanism. Microbiol Mol Biol Rev 70(3):605-45
6) Rybakin V and Clemen CS  (2005) Coronin proteins as multifunctional regulators of the cytoskeleton and membrane trafficking. Bioessays 27(6):625-32
7) Kreishman-Deitrick M and Rosen MK  (2002) Ignition of a cellular machine. Nat Cell Biol 4(2):E31-3
8) Rodal AA, et al.  (2005) Conformational changes in the Arp2/3 complex leading to actin nucleation. Nat Struct Mol Biol 12(1):26-31