SPP382/YLR424W Summary

SPP382 BASIC INFORMATION

Standard Name	SPP382 1, 2
Systematic Name	YLR424W
Alias	CCF8 3 , NTR1 4
Feature Type	ORF, Verified
Description	Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation (2, 3, 4)
Name Description	Suppressor of PrP38 #2 2

GO Annotations	All SPP382 GO evidence and references
	View Computational GO annotations for SPP382
Molecular Function
Manually curated	contributes_to ATP-dependent RNA helicase activity (IDA)
High-throughput	RNA binding (ISS)
Biological Process
Manually curated	generation of catalytic spliceosome for first transesterification step (IMP) spliceosome disassembly (IDA, IGI, IMP)
High-throughput	nuclear mRNA splicing, via spliceosome (IPI)
Cellular Component
Manually curated	spliceosomal complex (IDA) U2-type post-mRNA release spliceosomal complex (IDA, IMP)
High-throughput	cytoplasm (IDA) mitochondrion (IDA) nucleus (IDA)

Mutant Phenotype	All SPP382 Phenotype details and references
Classical genetics
null	inviable
reduction of function	unspliced RPS17A pre-mRNA accumulation: abnormal
Large-scale survey
conditional	heat sensitivity: increased
null	inviable

Interactions	SPP382 All interactions details and references
	150 total interaction(s) for 68 unique genes/features.
Physical Interactions	Affinity Capture-MS: 77 Affinity Capture-Western: 13 Biochemical Activity: 1 Co-fractionation: 2 PCA: 3 Reconstituted Complex: 1 Two-hybrid: 36
Genetic Interactions	Dosage Lethality: 9 Dosage Rescue: 3 Synthetic Growth Defect: 1 Synthetic Lethality: 2 Synthetic Rescue: 2

Sequence Information

ChrXII:973392 to 975518 | |

Last Update

Coordinates: 2006-01-09 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2127	973392..975518	2006-01-09	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004416

SPP382 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SPP382

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPP382]

1)	Lebaron S, et al. (2005) The splicing ATPase prp43p is a component of multiple preribosomal particles. Mol Cell Biol 25(21):9269-82
2)	Pandit S, et al. (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5
3)	Wang Q, et al. (2003) The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts. J Biol Chem 278(10):7875-83
4)	Tsai RT, et al. (2005) Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev 19(24):2991-3003

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