URA4/YLR420W Summary Help

Standard Name URA4 1
Systematic Name YLR420W
Feature Type ORF, Verified
Description Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate (1, 2 and see Summary Paragraph)
Name Description URAcil requiring 2
Chromosomal Location
ChrXII:963785 to 964879 | ORF Map | GBrowse
Gbrowse
Genetic position: 304 cM
Gene Ontology Annotations All URA4 GO evidence and references
  View Computational GO annotations for URA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 9 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
43 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • PCA: 3
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 25
  • Positive Genetic: 9
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 364
Molecular Weight (Da) 40,313
Isoelectric Point (pI) 6.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:963785 to 964879 | ORF Map | GBrowse
SGD ORF map
Genetic position: 304 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1095 963785..964879 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004412
SUMMARY PARAGRAPH for URA4

URA4 encodes dihydroorotase (DHOdehase), an enzyme involved in the de novo synthesis of pyrimidine ribonucleotides (shown here; 2). Ura4p catalyzes the third step in this pathway, the conversion of ureidosuccinic acid to dihydroorotate (2). Loss of Ura4p activity leads to an accumulation of ureidosuccinic acid (1) and a lack of cell growth unless uracil or uridine is added to the media (reviewed in 3).

Uracil starvation or increased levels of the pyrimidine biosynthesis pathway intermediate dihydoorotic acid (DHO) can induce URA4 expression three fold (4 and references contained therein). This regulation is mediated by the transcriptional activator Ppr1p, which binds to the UASURA site (CGGN6CCG) in the promoters of URA1, URA3, and URA4 (5). DNA-bound Ppr1p is transcriptionally inactive, but the addition of DHO converts Ppr1p to an active state that interacts with RNA polymerase II, leading to increased expression of the URA genes (5).

Last updated: 2005-12-16 Contact SGD

References cited on this page View Complete Literature Guide for URA4
1) Lacroute F  (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32
2) Guyonvarch A, et al.  (1988) Structure of the Saccharomyces cerevisiae URA4 gene encoding dihydroorotase. Mol Gen Genet 212(1):134-41
3) Jones ME  (1992) Orotidylate decarboxylase of yeast and man. Curr Top Cell Regul 33():331-42
4) Roy A, et al.  (1990) cis- and trans-acting regulatory elements of the yeast URA3 promoter. Mol Cell Biol 10(10):5257-70
5) Flynn PJ and Reece RJ  (1999) Activation of transcription by metabolic intermediates of the pyrimidine biosynthetic pathway. Mol Cell Biol 19(1):882-8