VIP1/YLR410W Summary Help

Standard Name VIP1 1
Systematic Name YLR410W
Feature Type ORF, Verified
Description Inositol hexakisphosphate and inositol heptakisphosphate kinase; inositol heptakisphosphate (IP7) production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1; inositol hexakisphosphate is also known as IP6 (1, 2, 3, 4 and see Summary Paragraph)
Chromosomal Location
ChrXII:937541 to 940981 | ORF Map | GBrowse
Gene Ontology Annotations All VIP1 GO evidence and references
  View Computational GO annotations for VIP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 3 genes
Classical genetics
Large-scale survey
270 total interaction(s) for 199 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 38
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 9
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 156
  • Phenotypic Suppression: 1
  • Positive Genetic: 37
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 3

Expression Summary
Length (a.a.) 1,146
Molecular Weight (Da) 129,754
Isoelectric Point (pI) 9.11
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:937541 to 940981 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3441 937541..940981 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004402

VIP1 encodes one of two yeast inositol pyrophosphate synthases (also known as inositol hexakisphophate kinases; IP6Ks); the other is encoded by KCS1. The inositol pyrophosphates (PP-IPs) produced by Kcs1p and/or Vip1p serve as high-energy signaling molecules involved in such diverse processes as vacuolar biogenesis, the stress response, DNA repair, cell wall synthesis, telomere maintenance, and phosphate homeostasis (see 5, 6, 7, and references therein).

Both enzymes catalyze the addition of beta-phosphate to the fully phosphorylated six-carbon ring of inositol hexakisphosphate (IP6). However, these enzymes produce different isomers of diphosphoinositol pentakisphosphate (IP7). Kcs1p phosphorylates IP6 at the C5 position forming 5PP-IP5, and Vip1p phosphorylates IP6 at the C4 or C6 position forming 4PP-IP5 or 6PP-IP5, respectively (the exact phosphorylation position has not yet been determined). The different IP7 isomers are biologically relevant: the Kcs1p product cannot substitute for the Vip1p product during phosphate homeostasis (2). Kcs1p and Vip1p also work in concert to produce bis-diphosphoinositol tetrakisphosphate ([PP]2-IP4; IP8). Kcs1p phosphorylates the Vip1p IP7 product 4/6PP-IP5, and Vip1p phosphorylates the Kcs1p IP7 product 5PP-IP5 (3).

Inositol pyrophosphate synthases are highly conserved and found across eukaryotes. In humans, three Kcs1p-like enzymes (IHPK1/IP6K1, IP6K2/IP6K2, IP6K3/IP6K3) (8, 9) and two Vip1p-like enzymes (HISPPD2a/PPIP5K1/VIP1 and PPIP5K2/VIP2) (10, 11) have been identified.

Last updated: 2008-01-22 Contact SGD

References cited on this page View Complete Literature Guide for VIP1
1) Feoktistova A, et al.  (1999) Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin. Genetics 152(3):895-908
2) Lee YS, et al.  (2007) Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science 316(5821):109-12
3) Mulugu S, et al.  (2007) A conserved family of enzymes that phosphorylate inositol hexakisphosphate. Science 316(5821):106-9
4) Pohlmann J and Fleig U  (2010) Asp1, a Conserved 1/3 Inositol Polyphosphate Kinase, Regulates the Dimorphic Switch in Schizosaccharomyces pombe. Mol Cell Biol 30(18):4535-47
5) Bennett M, et al.  (2006) Inositol pyrophosphates: metabolism and signaling. Cell Mol Life Sci 63(5):552-64
6) Onnebo SM and Saiardi A  (2007) Inositol pyrophosphates get the vip1 treatment. Cell 129(4):647-9
7) Bhandari R, et al.  (2007) Inositol pyrophosphate pyrotechnics. Cell Metab 5(5):321-3
8) Saiardi A, et al.  (1999) Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases. Curr Biol 9(22):1323-6
9) Saiardi A, et al.  (2001) Identification and characterization of a novel inositol hexakisphosphate kinase. J Biol Chem 276(42):39179-85
10) Fridy PC, et al.  (2007) Cloning and Characterization of Two Human VIP1-like Inositol Hexakisphosphate and Diphosphoinositol Pentakisphosphate Kinases. J Biol Chem 282(42):30754-62
11) Choi JH, et al.  (2007) Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress. J Biol Chem 282(42):30763-75