VPS33/YLR396C Summary Help

Standard Name VPS33 1, 2
Systematic Name YLR396C
Alias CLS14 3 , MET27 4 , PEP14 , SLP1 5 , 6 , VAM5 7 , VPL25 8 , VPT33 1
Feature Type ORF, Verified
Description ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains (9, 10, 11, 12, 13)
Name Description Vacuolar Protein Sorting 1, 2
Chromosomal Location
ChrXII:912310 to 910235 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All VPS33 GO evidence and references
  View Computational GO annotations for VPS33
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
179 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 52
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 13
  • Reconstituted Complex: 17
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 6
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 9
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 691
Molecular Weight (Da) 79,271
Isoelectric Point (pI) 5.02
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:912310 to 910235 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2076 912310..910235 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004388
References cited on this page View Complete Literature Guide for VPS33
1) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3) Ohya Y, et al.  (1986) Isolation and characterization of Ca2+-sensitive mutants of Saccharomyces cerevisiae. J Gen Microbiol 132(4):979-88
4) Jacquemin-Faure I, et al.  (1994) The vacuolar compartment is required for sulfur amino acid homeostasis in Saccharomyces cerevisiae. Mol Gen Genet 244(5):519-29
5) Kitamoto K, et al.  (1988) Mutants of Saccharomyces cerevisiae with defective vacuolar function. J Bacteriol 170(6):2687-91
6) Wada Y, et al.  (1990) The SLP1 gene of Saccharomyces cerevisiae is essential for vacuolar morphogenesis and function. Mol Cell Biol 10(5):2214-23
7) Wada Y, et al.  (1992) Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. I. Isolation and characterization of two classes of vam mutants. J Biol Chem 267(26):18665-70
8) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
9) Seals DF, et al.  (2000) A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion. Proc Natl Acad Sci U S A 97(17):9402-7
10) Gerhardt B, et al.  (1998) The vesicle transport protein Vps33p is an ATP-binding protein that localizes to the cytosol in an energy-dependent manner. J Biol Chem 273(25):15818-29
11) Darsow T, et al.  (1997) A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol 138(3):517-29
12) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
13) Lobingier BT and Merz AJ  (2012) Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex. Mol Biol Cell 23(23):4611-22