MDM30/YLR368W Summary Help

Standard Name MDM30 1
Systematic Name YLR368W
Alias DSG1 2
Feature Type ORF, Verified
Description F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains (2, 3, 4, 5, 6, 7)
Name Description Mitochondrial Distribution and Morphology 1
Chromosomal Location
ChrXII:857540 to 859336 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MDM30 GO evidence and references
  View Computational GO annotations for MDM30
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
63 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-Western: 5
  • Reconstituted Complex: 4
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 22
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 598
Molecular Weight (Da) 70,528
Isoelectric Point (pI) 6.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:857540 to 859336 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1797 857540..859336 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004360
References cited on this page View Complete Literature Guide for MDM30
1) Dimmer KS, et al.  (2002) Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell 13(3):847-53
2) Muratani M, et al.  (2005) The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell 120(6):887-99
3) Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
4) Fritz S, et al.  (2003) Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast. Mol Biol Cell 14(6):2303-13
5) Durr M, et al.  (2006) Nonredundant roles of mitochondria-associated F-box proteins Mfb1 and Mdm30 in maintenance of mitochondrial morphology in yeast. Mol Biol Cell 17(9):3745-55
6) Shukla A, et al.  (2009) Stimulation of mRNA export by an F-box protein, Mdm30p, in vivo. J Mol Biol 389(2):238-47
7) Cohen MM, et al.  (2011) Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion. J Cell Sci 124(Pt 9):1403-10