MDM30/YLR368W Summary

MDM30/YLR368W Summary

Standard Name	MDM30
Systematic Name	YLR368W
Alias	DSG1 1
Feature Type	ORF, Verified
Description	F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains (1, 2, 3, 4, 5, 6)
Name Description	Mitochondrial Distribution and Morphology 7

Chromosomal Location	ChrXII:857540 to 859336 \| ORF Map \| GBrowse

Gene Ontology Annotations All MDM30 GO evidence and references

	View Computational GO annotations for MDM30
Molecular Function
Manually curated	contributes_to ubiquitin-protein ligase activity (IMP, IPI)
Biological Process
Manually curated	chronological cell aging (IMP) mitochondrial fusion (IMP) mitochondrion organization (IMP) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IMP) proteolysis involved in cellular protein catabolic process (IDA, IMP) regulation of mRNA export from nucleus (IMP) SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IMP, IPI)
Cellular Component
Manually curated	mitochondrion (IPI) SCF ubiquitin ligase complex (IDA, IPI)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All MDM30 Phenotype evidence and references

Classical genetics
null	chronological lifespan: increased mitochondrial morphology: abnormal Fzo1p accumulation: increased Fzo1p modification: decreased resistance to hydrogen peroxide: increased respiratory metabolism: absent sporulation efficiency: decreased survival rate in stationary phase: increased
overexpression	budding: abnormal resistance to methylmercury chloride: normal
Large-scale survey
null	UV resistance: decreased competitive fitness: decreased filamentous growth: decreased haploinsufficient heat sensitivity: increased invasive growth: absent mitochondrial morphology: abnormal resistance to sirolimus: increased resistance to BPS: increased resistance to hydroxyurea: increased resistance to oleate: decreased resistance to ethanol: decreased resistance to methyl methanesulfonate: decreased resistance to wortmannin: increased respiratory growth: decreased rate sporulation: normal starvation resistance: decreased stress resistance: increased utilization of carbon source: absent viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MDM30 Interaction evidence and references

	60 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-Western: 4 Reconstituted Complex: 4 Two-hybrid: 11
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 2 Negative Genetic: 21 Phenotypic Enhancement: 2 Phenotypic Suppression: 4 Synthetic Growth Defect: 3 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MDM30 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:857540 to 859336 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1797	857540..859336	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004360

References cited on this page View Complete Literature Guide for MDM30

1)	Muratani M, et al. (2005) The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell 120(6):887-99
2)	Seol JH, et al. (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
3)	Fritz S, et al. (2003) Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast. Mol Biol Cell 14(6):2303-13
4)	Durr M, et al. (2006) Nonredundant roles of mitochondria-associated F-box proteins Mfb1 and Mdm30 in maintenance of mitochondrial morphology in yeast. Mol Biol Cell 17(9):3745-55
5)	Shukla A, et al. (2009) Stimulation of mRNA export by an F-box protein, Mdm30p, in vivo. J Mol Biol 389(2):238-47
6)	Cohen MM, et al. (2011) Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion. J Cell Sci 124(Pt 9):1403-10
7)	Dimmer KS, et al. (2002) Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell 13(3):847-53