RSC2/YLR357W Summary

RSC2/YLR357W Summary

Standard Name	RSC2
Systematic Name	YLR357W
Feature Type	ORF, Verified
Description	Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Remodel the Structure of Chromatin 6

Chromosomal Location	ChrXII:841331 to 844000 \| ORF Map \| GBrowse

Gene Ontology Annotations All RSC2 GO evidence and references

	View Computational GO annotations for RSC2
Molecular Function
Manually curated	contributes_to DNA translocase activity (IDA)
Biological Process
Manually curated	ATP-dependent chromatin remodeling (IDA) chromosome segregation (IGI) double-strand break repair via homologous recombination (IMP) double-strand break repair via nonhomologous end joining (IPI) nucleosome disassembly (IDA) plasmid maintenance (IMP) sister chromatid cohesion (IMP) transcription elongation from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	RSC complex (IDA)

Mutant phenotypes All RSC2 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal GFP-Rap1 distribution: abnormal POL30 modification: decreased Net1p modification: decreased resistance to 6-azauracil: decreased resistance to benomyl: decreased silencing: increased
Large-scale survey
null	auxotrophy bud morphology: abnormal cell size: increased glycogen accumulation: increased glutathione excretion: increased competitive fitness: decreased fermentative growth: decreased rate heat sensitivity: increased ionic stress resistance: decreased Fus-Mid-GFP distribution: abnormal resistance to hydroxyurea: decreased resistance to oleate: decreased resistance to nickel(2+): decreased resistance to sirolimus: decreased resistance to tunicamycin: increased resistance to mycophenolic acid: increased resistance to camptothecin: increased resistance to quinine: increased resistance to nickel sulfate: increased resistance to wortmannin: increased resistance to ethanol: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased resistance to tirapazamine: decreased resistance to cycloheximide: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to methyl methanesulfonate: decreased resistance to pentachlorophenol: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to dimethyl sulfoxide: decreased resistance to lovastatin: decreased resistance to 1,4-dithiothreitol: decreased resistance to sorbate: decreased stress resistance: increased telomere length: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RSC2 Interaction evidence and references

	416 total interaction(s) for 246 unique genes/features.
Physical Interactions	Affinity Capture-MS: 129 Affinity Capture-Western: 51 Co-fractionation: 1 Co-purification: 6 Reconstituted Complex: 5 Two-hybrid: 3
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 4 Negative Genetic: 64 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 88 Synthetic Growth Defect: 36 Synthetic Lethality: 21 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RSC2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:841331 to 844000 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2670	841331..844000	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004349

References cited on this page View Complete Literature Guide for RSC2

1)	Lorch Y, et al. (1998) Activated RSC-nucleosome complex and persistently altered form of the nucleosome. Cell 94(1):29-34
2)	Neely KE and Workman JL (2002) The complexity of chromatin remodeling and its links to cancer. Biochim Biophys Acta 1603(1):19-29
3)	Bungard D, et al. (2004) RSC1 and RSC2 are required for expression of mid-late sporulation-specific genes in Saccharomyces cerevisiae. Eukaryot Cell 3(4):910-8
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
6)	Cairns BR, et al. (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60