TAL1/YLR354C Summary Help

Standard Name TAL1 1
Systematic Name YLR354C
Feature Type ORF, Verified
Description Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication (1, 2 and see Summary Paragraph)
Name Description TransALdolase 1
Chromosomal Location
ChrXII:837357 to 836350 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All TAL1 GO evidence and references
  View Computational GO annotations for TAL1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
56 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 3
  • PCA: 1
  • Protein-peptide: 3

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 10
  • Phenotypic Enhancement: 2
  • Positive Genetic: 3
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 6
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 335
Molecular Weight (Da) 37,036
Isoelectric Point (pI) 6.36
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:837357 to 836350 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1008 837357..836350 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004346

Tal1p is a transaldolase (EC that is important for the non-oxidative branch of the pentose phosphate pathway (3) and is located in the cytosol (4). Lysine144 is essential for the catalytic activity of Tal1p (3). TAL1 encodes the only transaldolase in yeast, but is not an essential gene (1). Null mutants for tal1 accumulate sedoheptulose 7-phosphate, which is an intermediate of the pentose-phosphate pathway (1) and display increased sensitivity to hydrogen peroxide (5). TAL1 appears to be constitutively expressed under most conditions (1), but is repressed in response to dimethyl sulfoxide (DMSO) (6), and is induced in response to hydrogen peroxide in a Yap1p-dependent manner (5). It is also induced in response to xylose in a strain engineered to utilize xylose (7). Tal1p is of industrial interest because overproduction of Tal1p enhances the growth of recombinant S. cerevisiae strains engineered to ferment xylose (the major pentose in lignocellulose) to ethanol (8).

Tal1p has similarity to human TALDO1 (OMIM; 9), Candida utilis transaldolase (1) and Kluyveromyces lactis KITAL1, which can complement the transaldolase deficiency of tal1 null mutants (10).

Last updated: 2005-12-21 Contact SGD

References cited on this page View Complete Literature Guide for TAL1
1) Schaaff I, et al.  (1990) Molecular analysis of the structural gene for yeast transaldolase. Eur J Biochem 188(3):597-603
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Miosga T, et al.  (1993) Lysine144 is essential for the catalytic activity of Saccharomyces cerevisiae transaldolase. Yeast 9(11):1241-9
4) Maaheimo H, et al.  (2001) Central carbon metabolism of Saccharomyces cerevisiae explored by biosynthetic fractional (13)C labeling of common amino acids. Eur J Biochem 268(8):2464-79
5) Lee J, et al.  (1999) Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J Biol Chem 274(23):16040-6
6) Zhang W, et al.  (2003) Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide. J Ind Microbiol Biotechnol 30(1):57-69
7) Toivari MH, et al.  (2004) Endogenous xylose pathway in Saccharomyces cerevisiae. Appl Environ Microbiol 70(6):3681-6
8) Walfridsson M, et al.  (1995) Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase. Appl Environ Microbiol 61(12):4184-90
9) Banki K, et al.  (1994) Cloning and expression of the human gene for transaldolase. A novel highly repetitive element constitutes an integral part of the coding sequence. J Biol Chem 269(4):2847-51
10) Jacoby J, et al.  (1993) Transaldolase mutants in the yeast Kluyveromyces lactis provide evidence that glucose can be metabolized through the pentose phosphate pathway. Mol Microbiol 10(4):867-76