ORM2/YLR350W Summary Help

Standard Name ORM2 1
Systematic Name YLR350W
Feature Type ORF, Verified
Description Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Chromosomal Location
ChrXII:828730 to 829380 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ORM2 GO evidence and references
  View Computational GO annotations for ORM2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
611 total interaction(s) for 375 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Biochemical Activity: 8
  • Co-purification: 1
  • PCA: 32
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 411
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 16
  • Positive Genetic: 59
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 3
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 216
Molecular Weight (Da) 24,854
Isoelectric Point (pI) 9.09
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:828730 to 829380 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..651 828730..829380 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004342
References cited on this page View Complete Literature Guide for ORM2
1) Hjelmqvist L, et al.  (2002) ORMDL proteins are a conserved new family of endoplasmic reticulum membrane proteins. Genome Biol 3(6):RESEARCH0027
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Han S, et al.  (2010) Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality control. Proc Natl Acad Sci U S A 107(13):5851-6
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Shimobayashi M, et al.  (2013) TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis. Mol Biol Cell 24(6):870-81