MID2/YLR332W Summary

MID2 BASIC INFORMATION

Standard Name	MID2
Systematic Name	YLR332W
Alias	KAI1
Feature Type	ORF, Verified
Description	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p (1, 2)

GO Annotations	All MID2 GO evidence and references
	View Computational GO annotations for MID2
Molecular Function
Manually curated	transmembrane receptor activity (IGI, IMP, ISS)
Biological Process
Manually curated	cell morphogenesis during conjugation (IGI, IMP) fungal-type cell wall organization (IGI, IMP) response to acid (IMP) response to osmotic stress (IGI) UFP-specific transcription factor mRNA processing during unfolded protein response (IMP)
Cellular Component
Manually curated	integral to plasma membrane (IDA)
High-throughput	mating projection tip (IDA)

Mutant Phenotype	All MID2 Phenotype details and references
Classical genetics
null	resistance to (R)-(+)-alpha-terpineol: increased resistance to terpinen-4-ol: increased resistance to L-1,4-dithiothreitol: decreased resistance to mercaptoethanol: decreased resistance to tunicamycin: decreased
Large-scale survey
null	resistance to caspofungin: decreased viable

Interactions	MID2 All interactions details and references
	126 total interaction(s) for 83 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 3 PCA: 46 Protein-RNA: 2 Reconstituted Complex: 1 Two-hybrid: 2
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 31 Phenotypic Enhancement: 4 Synthetic Growth Defect: 3 Synthetic Lethality: 30 Synthetic Rescue: 3

Sequence Information

ChrXII:790676 to 791806 | |

Last Update

Coordinates: 2004-02-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1131	790676..791806	2004-02-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004324

MID2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for MID2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MID2]

1)	Philip B and Levin DE (2001) Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1. Mol Cell Biol 21(1):271-80
2)	Green R, et al. (2003) A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p, and identification of a Mid2p-interacting protein, Zeo1p, that modulates the PKC1-MPK1 cell integrity pathway. Microbiology 149(Pt 9):2487-99

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