CHS5/YLR330W Summary

CHS5/YLR330W Summary

Standard Name	CHS5
Systematic Name	YLR330W
Alias	CAL3
Feature Type	ORF, Verified
Description	Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane (1, 2, 3, 4, 5)
Name Description	CHitin Synthase-related 3

Chromosomal Location	ChrXII:787664 to 789679 \| ORF Map \| GBrowse

Gene Ontology Annotations All CHS5 GO evidence and references

	View Computational GO annotations for CHS5
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ascospore wall assembly (IMP) cell wall chitin catabolic process (IMP) cellular bud site selection (IMP) conjugation with cellular fusion (IMP) Golgi to plasma membrane transport (IGI, IMP) regulation of transcription, DNA-dependent (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) exomer complex (IDA)
High-throughput	mating projection tip (IDA)

Mutant phenotypes All CHS5 Phenotype evidence and references

Classical genetics
null	bipolar budding pattern: abnormal cell fusion: decreased tellurium atom accumulation: decreased chitin deposition: decreased cold sensitivity: increased fermentative growth: increased rate heat sensitivity: increased mating efficiency: decreased protein transport: abnormal Fus-Mid-GFP distribution: abnormal resistance to tellurite: increased resistance to Calcofluor White: increased viable
Large-scale survey
null	rubidium(1+) accumulation: decreased glutathione excretion: increased chitin deposition: decreased competitive fitness: decreased haploinsufficient heat sensitivity: increased hyperosmotic stress resistance: decreased ionic stress resistance: decreased mitochondrial genome maintenance: abnormal Fus-Mid-GFP distribution: abnormal resistance to paromomycin: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to Calcofluor White: increased resistance to fenpropimorph: increased resistance to tunicamycin: increased resistance to wortmannin: decreased resistance to hydroxyurea: decreased resistance to PM02734: decreased resistance to sirolimus: decreased resistance to quinine: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to sodium selenide: decreased resistance to mefloquine: decreased resistance to ciclopirox olamine: decreased resistance to clioquinol: decreased resistance to elesclomol-Cu: decreased resistance to calcium dichloride: decreased resistance to hygromycin B: decreased sporulation efficiency: decreased toxin resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CHS5 Interaction evidence and references

	421 total interaction(s) for 225 unique genes/features.
Physical Interactions	Affinity Capture-MS: 28 Affinity Capture-RNA: 2 Affinity Capture-Western: 11 Biochemical Activity: 1 Co-crystal Structure: 1 Co-localization: 1 Co-purification: 7 PCA: 10 Reconstituted Complex: 3 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 9 Negative Genetic: 152 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 11 Synthetic Growth Defect: 75 Synthetic Lethality: 104
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CHS5 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:787664 to 789679 | |

SGD ORF map

Last Update

Coordinates: 2004-02-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2016	787664..789679	2004-02-05	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004322

References cited on this page View Complete Literature Guide for CHS5

1)	Santos B, et al. (1997) CHS5, a gene involved in chitin synthesis and mating in Saccharomyces cerevisiae. Mol Cell Biol 17(5):2485-96
2)	Santos B and Snyder M (1997) Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p. J Cell Biol 136(1):95-110
3)	Cid VJ, et al. (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
4)	Trautwein M, et al. (2006) Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi. EMBO J 25(5):943-54
5)	Wang CW, et al. (2006) Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast. J Cell Biol 174(7):973-83