CWC24/YLR323C Summary Help

Standard Name CWC24 1
Systematic Name YLR323C
Feature Type ORF, Verified
Description General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p (1, 2, 3)
Name Description Complexed With Cef1p 1
Chromosomal Location
ChrXII:778952 to 778173 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CWC24 GO evidence and references
  View Computational GO annotations for CWC24
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
repressible
Large-scale survey
null
reduction of function
Resources
12 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 3
  • Biochemical Activity: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 259
Molecular Weight (Da) 29,741
Isoelectric Point (pI) 9.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:778952 to 778173 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..780 778952..778173 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004315
References cited on this page View Complete Literature Guide for CWC24
1) Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24
2) Ohrt T, et al.  (2012) Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. RNA 18(6):1244-56
3) Coltri PP and Oliveira CC  (2012) Cwc24p Is a General Saccharomyces cerevisiae Splicing Factor Required for the Stable U2 snRNP Binding to Primary Transcripts. PLoS One 7(9):e45678