Standard Name	VPS65
Systematic Name	YLR322W
Feature Type	ORF, Dubious
Description	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth (1, 2, 3)
Name Description	Vacuolar Protein Sorting 1

Chromosomal Location	ChrXII:777628 to 777942 | ORF Map | GBrowse

	View Computational GO annotations for VPS65
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
High-throughput	protein targeting to vacuole (IMP)
Cellular Component
Manually curated	cellular_component unknown (ND)

Classical genetics
null	metal resistance: decreased
Large-scale survey
null	alkaline pH resistance: decreased bud morphology: abnormal cell size: increased glycogen accumulation: decreased proton accumulation: decreased glutathione excretion: increased competitive fitness: decreased haploinsufficient heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased resistance to propan-1-ol: decreased resistance to methanol: decreased resistance to ethanol: decreased resistance to hydroxyurea: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to caspofungin: decreased resistance to oxalic acid: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to wortmannin: decreased resistance to sirolimus: decreased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to paromomycin: decreased resistance to streptomycin: decreased resistance to cadmium dichloride: decreased resistance to sorbate: decreased resistance to benomyl: decreased resistance to acetic acid: decreased resistance to hydrogen chloride: decreased resistance to amiodarone: decreased resistance to tunicamycin: decreased resistance to 1,4-dithiothreitol: decreased resistance to pentachlorophenol: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to sodium selenide: decreased resistance to dimethyl sulfoxide: decreased resistance to lovastatin: decreased stress resistance: decreased utilization of nitrogen source: absent vacuolar morphology: abnormal vegetative growth: decreased rate vegetative growth: absent viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:777628 to 777942 | ORF Map | GBrowse
Last Update	Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..315 777628..777942 2004-02-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004314