BUD6/YLR319C Summary Help

Standard Name BUD6
Systematic Name YLR319C
Alias AIP3 1 , 2
Feature Type ORF, Verified
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate (1, 3, 4, 5, 6)
Name Description BUD site selection
Chromosomal Location
ChrXII:771684 to 769318 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 221 cM
Gene Ontology Annotations All BUD6 GO evidence and references
  View Computational GO annotations for BUD6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 52 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
132 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Co-localization: 1
  • PCA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 34
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 7
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 788
Molecular Weight (Da) 88,816
Isoelectric Point (pI) 7.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:771684 to 769318 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 221 cM
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2367 771684..769318 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004311
References cited on this page View Complete Literature Guide for BUD6
1) Amberg DC, et al.  (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Biol Cell 8(4):729-53
2) Amberg DC, et al.  (1995) Defining protein interactions with yeast actin in vivo. Nat Struct Biol 2(1):28-35
3) Evangelista M, et al.  (1997) Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis. Science 276(5309):118-22
4) Graziano BR, et al.  (2011) Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor. Mol Biol Cell 22(21):4016-28
5) Tu D, et al.  (2012) Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6. Proc Natl Acad Sci U S A 109(50):E3424-33
6) Graziano BR, et al.  (2013) Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation. J Cell Biol 201(4):595-611