MEC3/YLR288C Summary Help

MEC3 BASIC INFORMATION

Standard Name MEC3
Systematic Name YLR288C
Alias PIP3 , PSO9 1
Feature Type ORF, Verified
Description DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 (2, 3, 4, 5, 6)
Name Description Mitosis Entry Checkpoint 7
GO Annotations All MEC3 GO evidence and references
    View Computational GO annotations for MEC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All MEC3 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions MEC3 All interactions details and references
136 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-Western: 11
  • Co-fractionation: 2
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 14
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 35
  • Synthetic Growth Defect: 37
  • Synthetic Lethality: 14
  • Synthetic Rescue: 7
Sequence Information
ChrXII:714906 to 713482 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1425 714906..713482 2004-02-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004279

MEC3 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for MEC3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MEC3]

1) Cardone JM, et al.  (2006) Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair (Amst) 5(2):163-71
2) Kondo T, et al.  (1999) Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Mol Cell Biol 19(2):1136-43
3) de la Torre-Ruiz M and Lowndes NF  (2000) The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining. FEBS Lett 467(2-3):311-5
4) Hong EJ and Roeder GS  (2002) A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev 16(3):363-76
5) Majka J and Burgers PM  (2003) Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A 100(5):2249-54
6) Rouse J and Jackson SP  (2002) Interfaces between the detection, signaling, and repair of DNA damage. Science 297(5581):547-51
7) Weinert TA, et al.  (1994) Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair. Genes Dev 8(6):652-65