YSH1/YLR277C Summary Help

Standard Name YSH1
Systematic Name YLR277C
Alias BRR5 1
Feature Type ORF, Verified
Description Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description Yeast Seventy-three kDa Homolog
Chromosomal Location
ChrXII:699495 to 697156 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All YSH1 GO evidence and references
  View Computational GO annotations for YSH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
overexpression
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
130 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 103
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 2
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 779
Molecular Weight (Da) 87,673
Isoelectric Point (pI) 4.95
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:699495 to 697156 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2340 699495..697156 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004267
References cited on this page View Complete Literature Guide for YSH1
1) Noble SM and Guthrie C  (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics 143(1):67-80
2) Chanfreau G, et al.  (1996) Essential yeast protein with unexpected similarity to subunits of mammalian cleavage and polyadenylation specificity factor (CPSF). Science 274(5292):1511-4
3) Wahle E and Ruegsegger U  (1999) 3'-End processing of pre-mRNA in eukaryotes. FEMS Microbiol Rev 23(3):277-95
4) Ryan K, et al.  (2004) Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. RNA 10(4):565-73
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Garas M, et al.  (2008) The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis. RNA 14(12):2671-2684
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76