YPT6/YLR262C Summary

Standard Name	YPT6 1
Systematic Name	YLR262C
Feature Type	ORF, Verified
Description	Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 (1, 2, 3 and see Summary Paragraph)
Name Description	Yeast Protein Two 1

Chromosomal Location	ChrXII:668891 to 668244 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All YPT6 GO evidence and references

	View Computational GO annotations for YPT6
Molecular Function
Manually curated	GTPase activity (IDA, ISS, TAS)
Biological Process
Manually curated	intracellular protein transport (IGI, TAS) retrograde transport, endosome to Golgi (IMP, TAS)
Cellular Component
Manually curated	Golgi apparatus (TAS)

Mutant phenotypes All YPT6 Phenotype evidence and references

Classical genetics
null	chronological lifespan: increased Acb1p secretion: absent Sys1p-GFP distribution: abnormal Imh1p-GFP distribution: abnormal Arl1p-GFP distribution: abnormal carboxypeptidase Y (Prc1p) modification: decreased resistance to gentamycin: decreased resistance to bortezomib: increased vacuolar morphology: abnormal
Large-scale survey
null	auxotrophy cell cycle progression in G1 phase: delayed rubidium(1+) accumulation: decreased glutathione excretion: increased inositol excretion: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploinsufficient heat sensitivity: increased metal resistance: decreased replicative lifespan: increased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to mefloquine: decreased resistance to monensin: decreased resistance to 4-chlorophenol: decreased resistance to propolis extract: decreased resistance to acetic acid: decreased resistance to pyocyanin: decreased resistance to (S)-lactic acid: decreased resistance to sirolimus: decreased resistance to wortmannin: normal resistance to caffeine: decreased resistance to L-canavanine: increased respiratory growth: decreased rate respiratory growth: decreased stress resistance: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YPT6 Interaction evidence and references

	1343 total interaction(s) for 740 unique genes/features.
Physical Interactions	Affinity Capture-MS: 19 Affinity Capture-RNA: 2 Affinity Capture-Western: 13 Biochemical Activity: 6 PCA: 3 Protein-peptide: 1 Reconstituted Complex: 6 Two-hybrid: 13
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 2 Dosage Rescue: 19 Negative Genetic: 706 Phenotypic Enhancement: 14 Phenotypic Suppression: 5 Positive Genetic: 288 Synthetic Growth Defect: 94 Synthetic Lethality: 146 Synthetic Rescue: 5
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YPT6 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:668891 to 668244 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..648	668891..668244	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004252

SUMMARY PARAGRAPH for YPT6

Rab proteins are small Ras-related GTPases that function in multiple stages in membrane traffic. Rabs continuously cycle between the cytosol and membranes. The GDP-bound form of the Rab is complexed with in the cytosol and are recruited to the membranes with the help of GDF (GDI Displacement Factor) and by their prenylation by geranylgeranyl transferase II (GGTase II). The membrane-bound Rab is activated to the GTP-bound form by the guanine nucleotide exchange factors (GEFs) which then goes on to interact with downstream effectors. The GTPase-activating protein (GAP) stimulates the hydrolysis of GTP to GDP to inactivate the Rab. Eleven Rabs have been identified in yeast and more than 60 in mammalian cells (reviewed in 4, 5, 6).

Last updated: 2010-05-18

References cited on this page View Complete Literature Guide for YPT6

1)	Li B and Warner JR (1996) Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J Biol Chem 271(28):16813-9
2)	Lazar T, et al. (1997) Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell? Trends Biochem Sci 22(12):468-72
3)	Luo Z and Gallwitz D (2003) Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem 278(2):791-9
4)	Cai H, et al. (2007) Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell 12(5):671-82
5)	Grosshans BL, et al. (2006) Rabs and their effectors: achieving specificity in membrane traffic. Proc Natl Acad Sci U S A 103(32):11821-7
6)	Bialek-Wyrzykowska U, et al. (2000) Low levels of Ypt protein prenylation cause vesicle polarization defects and thermosensitive growth that can be suppressed by genes involved in cell wall maintenance. Mol Microbiol 35(6):1295-311