HAP1/YLR256W Summary Help

Standard Name HAP1 1, 2
Systematic Name YLR256W
Alias CYP1 3
Feature Type ORF, Verified
Description Zinc finger transcription factor; involved in the complex regulation of gene expression in response to levels of heme and oxygen; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus (2, 4, 5, 6, 7)
Name Description Heme Activator Protein 1, 2
Chromosomal Location
ChrXII:646415 to 650923 | ORF Map | GBrowse
Gene Ontology Annotations All HAP1 GO evidence and references
  View Computational GO annotations for HAP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 75 genes
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
35 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 2

Genetic Interactions
  • Phenotypic Enhancement: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,502
Molecular Weight (Da) 166,106
Isoelectric Point (pI) 7.35
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:646415 to 650923 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4509 646415..650923 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004246
References cited on this page View Complete Literature Guide for HAP1
1) Guarente L, et al.  (1984) Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36(2):503-11
2) Pfeifer K, et al.  (1989) Functional dissection and sequence of yeast HAP1 activator. Cell 56(2):291-301
3) Verdiere J, et al.  (1985) Regulation of the expression of iso 2-cytochrome c gene in S. cerevisiae: cloning of the positive regulatory gene CYP1 and identification of the region of its target sequence on the structural gene CYP3. Mol Gen Genet 199(3):524-33
4) Keng T  (1992) HAP1 and ROX1 form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol Cell Biol 12(6):2616-23
5) Pfeifer K, et al.  (1987) Yeast HAP1 activator binds to two upstream activation sites of different sequence. Cell 49(1):19-27
6) Gaisne M, et al.  (1999) A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1). Curr Genet 36(4):195-200
7) Kwast KE, et al.  (1998) Oxygen sensing and the transcriptional regulation of oxygen-responsive genes in yeast. J Exp Biol 201(Pt 8):1177-95
8) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
9) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11
10) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104