RCK2/YLR248W Summary Help

Standard Name RCK2 1
Systematic Name YLR248W
Alias CLK1 2 , CMK3 3
Feature Type ORF, Verified
Description Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication (2, 4, 5, 6, 7)
Name Description Radiation sensitivity Complementing Kinase 1
Chromosomal Location
ChrXII:634252 to 636084 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RCK2 GO evidence and references
  View Computational GO annotations for RCK2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
105 total interaction(s) for 79 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Biochemical Activity: 28
  • PCA: 3
  • Reconstituted Complex: 5
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 9
  • Negative Genetic: 12
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 2
  • Synthetic Haploinsufficiency: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 610
Molecular Weight (Da) 68,061
Isoelectric Point (pI) 5.18
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:634252 to 636084 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1833 634252..636084 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004238
References cited on this page View Complete Literature Guide for RCK2
1) Dahlkvist A and Sunnerhagen P  (1994) Two novel deduced serine/threonine protein kinases from Saccharomyces cerevisiae. Gene 139(1):27-33
2) Melcher ML and Thorner J  (1996) Identification and characterization of the CLK1 gene product, a novel CaM kinase-like protein kinase from the yeast Saccharomyces cerevisiae. J Biol Chem 271(47):29958-68
3) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
4) Dahlkvist A, et al.  (1995) The RCK1 and RCK2 protein kinase genes from Saccharomyces cerevisiae suppress cell cycle checkpoint mutations in Schizosaccharomyces pombe. Mol Gen Genet 246(3):316-26
5) Bilsland E, et al.  (2004) Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance. Mol Microbiol 53(6):1743-56
6) Teige M, et al.  (2001) Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast. Proc Natl Acad Sci U S A 98(10):5625-30
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61