IRC20/YLR247C Summary Help

Standard Name IRC20 1
Systematic Name YLR247C
Feature Type ORF, Verified
Description E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci (1, 2, 3, 4, 5)
Name Description Increased Recombination Centers 1
Chromosomal Location
ChrXII:633354 to 628684 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IRC20 GO evidence and references
  View Computational GO annotations for IRC20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 16 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
44 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 1
  • Co-purification: 1

Genetic Interactions
  • Negative Genetic: 21
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,556
Molecular Weight (Da) 180,342
Isoelectric Point (pI) 6.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:633354 to 628684 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4671 633354..628684 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004237
References cited on this page View Complete Literature Guide for IRC20
1) Alvaro D, et al.  (2007) Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination. PLoS Genet 3(12):e228
2) Shiratori A, et al.  (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Sickmann A, et al.  (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
5) Richardson A, et al.  (2013) Physical and Genetic Associations of the Irc20 Ubiquitin Ligase with Cdc48 and SUMO. PLoS One 8(10):e76424