YKE2/YLR200W Summary Help

Standard Name YKE2 1
Systematic Name YLR200W
Alias GIM1 , PFD6
Feature Type ORF, Verified
Description Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation (2, 3, 4)
Name Description Yeast ortholog of mouse KE2 1
Chromosomal Location
ChrXII:549012 to 549356 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All YKE2 GO evidence and references
  View Computational GO annotations for YKE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
918 total interaction(s) for 465 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 4
  • PCA: 7
  • Two-hybrid: 37

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 388
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 7
  • Positive Genetic: 164
  • Synthetic Growth Defect: 169
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 89
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 114
Molecular Weight (Da) 13,284
Isoelectric Point (pI) 4.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:549012 to 549356 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..345 549012..549356 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004190
References cited on this page View Complete Literature Guide for YKE2
1) Shang HS, et al.  (1994) YKE2, a yeast nuclear gene encoding a protein showing homology to mouse KE2 and containing a putative leucine-zipper motif. Gene 151(1-2):197-201
2) Geissler S, et al.  (1998) A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J 17(4):952-66
3) Siegers K, et al.  (1999) Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system. EMBO J 18(1):75-84
4) Millan-Zambrano G, et al.  (2013) The Prefoldin Complex Regulates Chromatin Dynamics during Transcription Elongation. PLoS Genet 9(9):e1003776