HCR1/YLR192C Summary Help

Standard Name HCR1 1
Systematic Name YLR192C
Feature Type ORF, Verified
Description eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for processing of 20S pre-rRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA (1, 2, 3)
Name Description High-Copy suppressor of Rpg1 1
Chromosomal Location
ChrXII:539589 to 538792 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All HCR1 GO evidence and references
  View Computational GO annotations for HCR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
195 total interaction(s) for 134 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 15
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Protein-peptide: 6
  • Reconstituted Complex: 4
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 6
  • Negative Genetic: 42
  • Phenotypic Suppression: 2
  • Positive Genetic: 47
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 265
Molecular Weight (Da) 29,564
Isoelectric Point (pI) 4.86
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:539589 to 538792 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..798 539589..538792 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004182
References cited on this page View Complete Literature Guide for HCR1
1) Valasek L, et al.  (1999) The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3. J Biol Chem 274(39):27567-72
2) Phan L, et al.  (2001) A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J 20(11):2954-65
3) Risler JK, et al.  (2012) Host co-factors of the retrovirus-like transposon Ty1. Mob DNA 3(1):12