TOS4/YLR183C Summary Help

Standard Name TOS4 1
Systematic Name YLR183C
Feature Type ORF, Verified
Description Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase (HDAC) complexes; APCC(Cdh1) substrate; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Target Of Sbf 1
Chromosomal Location
ChrXII:522012 to 520543 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All TOS4 GO evidence and references
  View Computational GO annotations for TOS4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
40 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 5
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 18
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 489
Molecular Weight (Da) 55,467
Isoelectric Point (pI) 10.12
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:522012 to 520543 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1470 522012..520543 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004173
References cited on this page View Complete Literature Guide for TOS4
1) Horak CE, et al.  (2002) Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae. Genes Dev 16(23):3017-33
2) de Lichtenberg U, et al.  (2005) New weakly expressed cell cycle-regulated genes in yeast. Yeast 22(15):1191-201
3) Tu BP, et al.  (2005) Logic of the yeast metabolic cycle: temporal compartmentalization of cellular processes. Science 310(5751):1152-8
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Huang M and Elledge SJ  (2000) The FHA domain, a phosphoamino acid binding domain involved in the DNA damage response pathway. Cold Spring Harb Symp Quant Biol 65():413-21
6) Bastos de Oliveira FM, et al.  (2012) Linking DNA replication checkpoint to MBF cell-cycle transcription reveals a distinct class of G1/S genes. EMBO J 31(7):1798-810
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Ostapenko D, et al.  (2012) Identification of anaphase promoting complex substrates in S. cerevisiae. PLoS One 7(9):e45895