STM1/YLR150W Summary Help

Standard Name STM1 1
Systematic Name YLR150W
Alias MPT4 2
Feature Type ORF, Verified
Description Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery (1, 3, 4, 5, 6, 7, 8, 9)
Name Description Suppressor of ToM1 1
Chromosomal Location
ChrXII:440467 to 441288 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All STM1 GO evidence and references
  View Computational GO annotations for STM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
249 total interaction(s) for 222 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 143
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 1
  • Biochemical Activity: 5
  • PCA: 3
  • Protein-peptide: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Lethality: 3
  • Dosage Rescue: 18
  • Negative Genetic: 39
  • Positive Genetic: 9
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 273
Molecular Weight (Da) 29,995
Isoelectric Point (pI) 10.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:440467 to 441288 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..822 440467..441288 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004140
References cited on this page View Complete Literature Guide for STM1
1) Utsugi T, et al.  (1995) A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochim Biophys Acta 1263(3):285-8
2) Frantz JD and Gilbert W  (1995) A yeast gene product, G4p2, with a specific affinity for quadruplex nucleic acids. J Biol Chem 270(16):9413-9
3) Nelson LD, et al.  (2000) The yeast STM1 gene encodes a purine motif triple helical DNA-binding protein. J Biol Chem 275(8):5573-81
4) Hayashi N and Murakami S  (2002) STM1, a gene which encodes a guanine quadruplex binding protein, interacts with CDC13 in Saccharomyces cerevisiae. Mol Genet Genomics 267(6):806-13
5) Van Dyke MW, et al.  (2004) Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae. J Biol Chem 279(23):24323-33
6) Van Dyke N, et al.  (2006) Stm1p, a ribosome-associated protein, is important for protein synthesis in Saccharomyces cerevisiae under nutritional stress conditions. J Mol Biol 358(4):1023-31
7) Van Dyke N, et al.  (2009) Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation. Nucleic Acids Res 37(18):6116-25
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Van Dyke N, et al.  (2013) The Saccharomyces cerevisiae protein Stm1p facilitates ribosome preservation during quiescence. Biochem Biophys Res Commun 430(2):745-50