PEP3/YLR148W Summary Help

Standard Name PEP3 1
Systematic Name YLR148W
Alias VAM8 , VPS18 2 , 3 , VPT18 2
Feature Type ORF, Verified
Description Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis (4, 5, 6, 7)
Name Description carboxyPEPtidase Y-deficient 8
Chromosomal Location
ChrXII:434641 to 437397 | ORF Map | GBrowse
Gbrowse
Genetic position: 91 cM
Gene Ontology Annotations All PEP3 GO evidence and references
  View Computational GO annotations for PEP3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All PEP3 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All PEP3 Interaction evidence and references
139 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 27
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 19
Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 4
  • Phenotypic Enhancement: 7
  • Synthetic Growth Defect: 18
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All PEP3 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:434641 to 437397 | ORF Map | GBrowse
SGD ORF map
Genetic position: 91 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2757 434641..437397 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004138
References cited on this page View Complete Literature Guide for PEP3
1) Jones, E.  (1985) Personal Communication, Mortimer Map Edition 9
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Srivastava A, et al.  (2000) Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae. Genetics 156(1):105-22
5) Preston RA, et al.  (1991) Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(12):5801-12
6) Robinson JS, et al.  (1991) A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. Mol Cell Biol 11(12):5813-24
7) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
8) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33