PEP3/YLR148W Summary Help

Standard Name PEP3 1
Systematic Name YLR148W
Alias VAM8 , VPS18 2 , 3 , VPT18 2
Feature Type ORF, Verified
Description Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis (4, 5, 6, 7, 8)
Name Description carboxyPEPtidase Y-deficient 9
Chromosomal Location
ChrXII:434641 to 437397 | ORF Map | GBrowse
Genetic position: 91 cM
Gene Ontology Annotations All PEP3 GO evidence and references
  View Computational GO annotations for PEP3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
141 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 27
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 19

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 4
  • Phenotypic Enhancement: 7
  • Synthetic Growth Defect: 18
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 918
Molecular Weight (Da) 107,397
Isoelectric Point (pI) 6.94
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:434641 to 437397 | ORF Map | GBrowse
Genetic position: 91 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2757 434641..437397 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004138
References cited on this page View Complete Literature Guide for PEP3
1) Jones, E.  (1985) Personal Communication, Mortimer Map Edition 9
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Srivastava A, et al.  (2000) Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae. Genetics 156(1):105-22
5) Preston RA, et al.  (1991) Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11(12):5801-12
6) Robinson JS, et al.  (1991) A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation. Mol Cell Biol 11(12):5813-24
7) Peplowska K, et al.  (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
8) Balderhaar HJ, et al.  (2013) The CORVET complex promotes tethering and fusion of Rab5/Vps21-positive membranes. Proc Natl Acad Sci U S A 110(10):3823-8
9) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33