SLX4/YLR135W Summary Help

Standard Name SLX4 1
Systematic Name YLR135W
Feature Type ORF, Verified
Description Endonuclease involved in processing DNA; acts during recombination and repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress (2, 3, 4, 5, 6)
Name Description Synthetic Lethal of unknown (X) function 1
Chromosomal Location
ChrXII:413281 to 415527 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SLX4 GO evidence and references
  View Computational GO annotations for SLX4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
298 total interaction(s) for 217 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-Western: 12
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 3
  • Negative Genetic: 155
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 4
  • Positive Genetic: 33
  • Synthetic Growth Defect: 35
  • Synthetic Lethality: 19
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 748
Molecular Weight (Da) 84,361
Isoelectric Point (pI) 4.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:413281 to 415527 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2247 413281..415527 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004125
References cited on this page View Complete Literature Guide for SLX4
1) Mullen JR, et al.  (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157(1):103-18
2) Fricke WM and Brill SJ  (2003) Slx1-Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1-Top3. Genes Dev 17(14):1768-78
3) Li F, et al.  (2008) Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates. Mol Cell 30(3):325-35
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Ward TA, et al.  (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884
6) Pardo B and Aguilera A  (2012) Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases. PLoS Genet 8(9):e1002979