Standard Name	DCN1 1
Systematic Name	YLR128W
Feature Type	ORF, Verified
Description	Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation (1, 2)
Name Description	Defective in Cullin Neddylation 1

Chromosomal Location	ChrXII:398530 to 399433 | ORF Map | GBrowse

Molecular Function
Manually curated	NEDD8 ligase activity (IDA, IMP) small conjugating protein binding (IDA) ubiquitin binding (IDA)
Biological Process
Manually curated	positive regulation of ubiquitin-protein ligase activity (IMP, IPI) protein neddylation (IDA, IGI, IMP)
Cellular Component
Manually curated	cellular_component unknown (ND)

Classical genetics
null	Lag2p modification: decreased Cdc53p modification: decreased viable
overexpression	Cdc53p modification: increased
reduction of function	Cdc53p modification: decreased
Large-scale survey
null	competitive fitness: increased haploproficient toxin resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	40 total interaction(s) for 23 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Biochemical Activity: 2 Co-crystal Structure: 2 Co-fractionation: 1 Co-purification: 1 Reconstituted Complex: 10 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 10 Phenotypic Suppression: 1 Positive Genetic: 2 Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:398530 to 399433 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3 398530..398532 2011-02-03 1996-07-31 Intron 4..97 398533..398626 2011-02-03 1996-07-31 CDS 98..904 398627..399433 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS) Intron (4 - 97)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004118