DCN1/YLR128W Summary Help

Standard Name DCN1 1
Systematic Name YLR128W
Feature Type ORF, Verified
Description Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation (1, 2)
Name Description Defective in Cullin Neddylation 1
Chromosomal Location
ChrXII:398530 to 399433 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All DCN1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
Resources
41 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 2
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 2
  • Reconstituted Complex: 10
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 10
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 269
Molecular Weight (Da) 32,203
Isoelectric Point (pI) 4.6
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:398530 to 399433 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3 398530..398532 2011-02-03 1996-07-31
Intron 4..97 398533..398626 2011-02-03 1996-07-31
CDS 98..904 398627..399433 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004118
References cited on this page View Complete Literature Guide for DCN1
1) Kurz T, et al.  (2005) The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae. Nature 435(7046):1257-61
2) Kurz T, et al.  (2008) Dcn1 functions as a scaffold-type e3 ligase for cullin neddylation. Mol Cell 29(1):23-35