CFT2/YLR115W Summary Help

Standard Name CFT2 1
Systematic Name YLR115W
Alias YDH1 2
Feature Type ORF, Verified
Description Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. (1, 2, 3)
Name Description Cleavage Factor Two
Chromosomal Location
ChrXII:377985 to 380564 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CFT2 GO evidence and references
  View Computational GO annotations for CFT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Large-scale survey
conditional
null
reduction of function
Resources
119 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 94
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Co-purification: 5
  • Reconstituted Complex: 9
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 859
Molecular Weight (Da) 96,253
Isoelectric Point (pI) 7.62
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:377985 to 380564 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2580 377985..380564 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004105
References cited on this page View Complete Literature Guide for CFT2
1) Zhao J, et al.  (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8
2) Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
3) Kyburz A, et al.  (2003) The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation. Nucleic Acids Res 31(14):3936-45