CDC45/YLR103C Summary

CDC45 BASIC INFORMATION

Standard Name	CDC45 1
Systematic Name	YLR103C
Alias	SLD4
Feature Type	ORF, Verified
Description	DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes (2, 3)
Name Description	Cell Division Cycle 1

GO Annotations	All CDC45 GO evidence and references
	View Computational GO annotations for CDC45
Molecular Function
Manually curated	chromatin binding (IDA) DNA replication origin binding (IDA)
Biological Process
Manually curated	DNA replication initiation (IGI, IMP) pre-replicative complex assembly (IPI) regulation of chromatin silencing at telomere (IMP) S phase of mitotic cell cycle (IGI, IMP)
Cellular Component
Manually curated	DNA replication preinitiation complex (IDA) nuclear replication fork (IDA) nucleus (IDA) pre-replicative complex (IDA) replication fork protection complex (IDA)

Mutant Phenotype	All CDC45 Phenotype details and references
Classical genetics
conditional	cell cycle progression: arrested cell cycle progression in S phase: arrested chromosome segregation: abnormal cold sensitivity: increased mitotic recombination: increased
null	inviable
Large-scale survey
null	inviable
repressible	cell cycle progression in S phase: abnormal

Interactions	CDC45 All interactions details and references
	161 total interaction(s) for 117 unique genes/features.
Physical Interactions	Affinity Capture-MS: 18 Affinity Capture-Western: 24 Co-fractionation: 1 Co-localization: 3 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 3 Synthetic Growth Defect: 2 Synthetic Lethality: 105

Sequence Information

ChrXII:345942 to 343990 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1953	345942..343990	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004093

CDC45 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for CDC45

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CDC45]

1)	Moir D, et al. (1982) Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100(4):547-63
2)	Tye BK (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
3)	McKie JM, et al. (1998) Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene. Genome Res 8(8):834-41

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