ERG27/YLR100W Summary

Standard Name	ERG27
Systematic Name	YLR100W
Feature Type	ORF, Verified
Description	3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs (1, 2, 3, 4 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis

Chromosomal Location	ChrXII:341810 to 342853 \| ORF Map \| GBrowse

Gene Ontology Annotations All ERG27 GO evidence and references

	View Computational GO annotations for ERG27
Molecular Function
Manually curated	3-keto sterol reductase activity (IMP)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) endoplasmic reticulum membrane (IDA)
High-throughput	mitochondrial outer membrane (IDA)

Pathways	ergosterol biosynthesis superpathway of ergosterol biosynthesis

Mutant phenotypes All ERG27 Phenotype evidence and references

Classical genetics
null	auxotrophy inviable
Large-scale survey
conditional	heat sensitivity: increased
null	inviable vegetative growth: normal
reduction of function	resistance to hydroxyurea: decreased
repressible	mitochondrial morphology: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG27 Interaction evidence and references

	61 total interaction(s) for 25 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 27 PCA: 22 Two-hybrid: 4
Genetic Interactions	Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG27 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:341810 to 342853 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1044	341810..342853	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004090

SUMMARY PARAGRAPH for ERG27

ERG27 encodes 3-keto sterol reductase, an enzyme that catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis (3, 4, 1, 2). Cells lacking ERG27 are sterol auxotrophs, and accumulate noncyclic sterol intermediates when grown in the presence of sterols (3).

Last updated: 2000-09-01

References cited on this page View Complete Literature Guide for ERG27

1)	Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
2)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3)	Gachotte D, et al. (1999) Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc Natl Acad Sci U S A 96(22):12655-60
4)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press